The topic of our round-table workshop on February 27, 2025, at 4:00 PM ET USA was Suppress or not to Suppress?!… – CRAFT it ! – Extracting essential biomarker signals directly from the full 1H NMR spectrum of existing serum samples.
The panel introduced a very efficient and powerful software tool, CRAFT (Complete Reduction to Amplitude-Frequency Table) to extract essential biomarker signals from horse serum 1H spectra, namely N+Me signals of lipoproteins (GlycA and GlycB) and a composite signal of phospho-glycoproteins (SPC), which are especially indicative of inflammatory conditions. It is an orthogonal approach to the sophisticated, yet somewhat costly experimental suppression techniques (DIRE, JEDI).
Our approach uses the full 1D spectrum (only water suppression applied). Partial overlap with other signals or broad background resonances can be efficiently handled. A special advantage of this approach that it can be used retrospectively on any full spectra recorded earlier.
We applied the CRAFT protocol to 20+20 horse serum samples of equal number of healthy and diseased subjects diagnosed with OCD (Osteochondritis Dissecans), respectively, as a case study. The integral ratios of the Gly and SPC signals clearly identify the diseased cohort with good validation in O-PLS-DA. Similar analyses of various datasets from earlier studies are also presented, a study of falcon blood samples to diagnosed with a respiratory disease, and that of samples from horses diagnosed with laminitis.
Panelists: Istvan Pelczer, James Chen, Christina Metaxas, Krish Krishnamurthy